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KMID : 0379120040320020125
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Korean Journal of Mycology
2004 Volume.32 No. 2 p.125 ~ p.129
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Purification and Characterization of Carboxymethyl Cellulase from Lampteromyces japonicus
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Yoo Kwan-Hee
Kim Jun-Ho
Jang Hyung-Soo
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Abstract
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A carboxymethyl cellulase (CMCase) bas been isolated and purified from Lampteromyces japonicus. The molecular weight of CMCase was estimated to be 42 kDa by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The maximum activity of the purified CMCase was observed at pH 6.0 and $30^{\circ}C$, and stable for pH 4 to 7 to maintain 40% activity. The CMCase activity was activated by $Al_{2}(SO_{4})_{3}$, and inhibited by SDS. Also, the enzyme activity was decreased by the addition of ethylene diamine tetraacetic acid (EDTA), suggesting that the purified CMCase is metalloenzyme.
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KEYWORD
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CMCase, Lampteromyces japonicus, pH
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